Microbiology Lab

Unknown Final report 1. Introduction: The purpose of this laboratory was to spot two unfathomable bacterium cultures using several(a) derivative bear witnesss.   The identification of these obscure cultures was accomplished by separating and differentiating possible bacteria based on specialized biochemical characteristics.   Whether the adjudicates performed place specific enzymatic reactions or metabolic pathways, each(prenominal) was use in a way to help discover those specifics and identify the unknown cultures.   The differential quizs used to identify the unknown cultures were oxidase, catalase, lactose and saccharose fermentation, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, indole, urease, DNase, and coagulase.   The tests performed on the unknown bacteria cultures were all used to determine the individuality of the bacteria.   Each of the tests performed provided most key information about the bacteria in head word and how it functions.   All of the tests were performed on either culture, however, as some of the tests were used only for gram (+) or gram (-) bacteria, while others were nevertheless more specific and identified only for cocci bacteria.   The tests performed and what constitutes a positive and negative test are as follows. 2.

tryout Result of all organism similar to my unknown get hold following(a) pages 3. Flow chart See page with flow chart 4. Test Result Oxidase This scrutiny ground test is based on detecting the doing of the enzyme cytochrome oxidase by Gram-ne gative bacteria if no color mixture to pur! ple or blue is evident at 30 seconds-1 slight then the result is positive. It is important that the test is represent by one minute to ensure accurate results (avoid trumped-up(prenominal) negatives and fancied positives).  Unknown 12 resulted in a negative test with no color change. These were similar results seen in Eschericia coli, Proteus vulgaris, genus genus Serratia marcescens, and Enterobacter aerogenes. This test helped to rule out two of the six unknown...If you need to get a full essay, order it on our website: BestEssayCheap.com

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